ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
Non-polar molecules are slowed down on their way with the column. They sort varying levels of attraction Along with the hydrocarbon groups principally by means of van der Waals dispersion forces and hydrophobic interactions.
. The working cylinder as well as the equilibrating cylinder for your pump within the still left choose solvent from reservoir A and send it to the mixing chamber. The pump on the best moves solvent from reservoir B to your mixing chamber.
Peak parts: The world less than Every single peak in the chromatogram is proportional to the level of analyte existing, letting for quantification.
各種の高速液体クロマトグラフィーの項目にある違いは、カラムの違いである事が多いため、装置はそのままでカラムの変更で行える場合が有る。ただし、誤って不適当な溶媒を通すとカラムを破損することがあるため、切り替えを行う際には注意が必要である。
The elution buy of solutes in HPLC is governed by polarity. For a normal-period separation, a solute of lessen polarity spends proportionally fewer time while in the polar stationary section and elutes ahead of a solute which is additional polar. Presented a selected stationary section, retention moments in website ordinary-stage HPLC are controlled by adjusting the cell section’s Homes. For instance, if the resolution amongst two solutes is very poor, switching to some a lot less polar mobile section retains the solutes within the column for an extended time and supplies additional option for his or her separation.
). As the tubing and fittings that carry the mobile phase have stress boundaries, a higher back stress needs a lessen circulation amount and a longer Assessment time. Monolithic columns, by which the reliable help is a single, porous rod, offer you column efficiencies such as a packed capillary column while letting for more quickly move charges. A monolithic column—which commonly is analogous in dimensions to a standard packed column, Despite the fact that smaller, capillary columns also are available—is ready by forming the mono- lithic rod in a mold and masking it with PTFE tubing or possibly a polymer resin.
移動相としては、カラムや装置に悪影響を与えない範囲で各種の溶媒が使用される。水や塩類の水溶液、アルコール類、アセトニトリル、ジクロロメタン、トリフルオロ酢酸などが用いられる。相溶性のある（互いに混じり合う）溶媒を混合して使用する場合が多い。
Fluoxetine is another title with the antidepressant drug Prozac. The dedication of fluoxetine in serum is a crucial Portion of checking its therapeutic use.
From the ionization chamber the remaining molecules—a combination of the cellular period factors and solutes—go through ionization and fragmentation. The mass spectrometer’s mass analyzer separates the ions by their mass-to-cost ratio (m/z). A detector counts the ions and shows the mass spectrum.
Column assortment: The stationary period during the column interacts with analytes. Using the Mistaken column chemistry can here result in weak resolution. Consider using a different column by using a stationary section that provides improved selectivity for the analytes.
The separation of the person components in the combination usually takes place during the stationary stage inside the column. As opposed to the glass column, it is ready in chrome steel.